上海恒遠(yuǎn)生物和華東師范大學(xué)多次合作帶來的合作成果！

恒遠(yuǎn)產(chǎn)品文獻(xiàn):大鼠胰島素,瘦素,游離脂肪酸ELISA試劑盒引用文獻(xiàn)

【文獻(xiàn)標(biāo)題】Hyperlipidemia induces typical atherosclerosis development in Ldlr and Apoe deficient rats

【作者】Yongliang Zhao, Yiqing Yang, Roumei Xing，et.al

【作者單位】華東師范大學(xué)（East China Normal University）

【文獻(xiàn)中引用產(chǎn)品】

大鼠胰島素(INS)ELISA試劑盒

大鼠瘦素(LEP)ELISA試劑盒

大鼠游離脂肪酸(FFA)ELISA試劑盒

【關(guān)鍵詞】 Apoe; Ldl receptor; atherosclerosis; rat; gene knockout 

【影響因子(IF)】4.26

【出版期刊】《Atherosclerosis》

【產(chǎn)品原文引用】

Biochemical analysis 

103 Rats were fasted overnight (12-14 h) and blood samples from the retro-orbital plexus were 

104 collected. Serum was obtained by centrifugation at 3000 rpm for 15 min at 4 °C, then kept 

105 frozen at ?80 °C until analysis. Lipids and lipoproteins including total cholesterol (TC), 

106 triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein 

107 cholesterol (HDL-C), ApoB and lipoprotein (a) were analyzed using AU680 Automatic

108 Biochemistry Analyzer (Beckman Coulter, USA). FPLC lipoprotein profiles were assessed by 

109 size-exclusion chromatography on Superose 6 10/300 GL column and AKTA purifier (GE 

110 Healthcare) [16]. We also measured liver and kidney indexes such as aspartate 

111 aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA) and creatinine levels. 

112 Furthermore, the atherosclerosis index and LDL/HDL ratio were calculated. Atherosclerosis 

113 index was calculated as [TC- HDL-C]/HDL-C [17]. Serum insulin, leptin and free fatty acid 

114 (FFA) levels were measured using ELISA kits (Hengyuan Biological Technology Co. Ltd., 

115 Shanghai, China

恒遠(yuǎn)產(chǎn)品文獻(xiàn):魚丙二醛（MDA）ELISA試劑盒引用文獻(xiàn)

【文獻(xiàn)標(biāo)題】Environmental concentrations of antibiotics impair zebrafish gut health

【作者】Li Zhou，Samwel Mchele Limbu，Meilin Shen，et.al

【作者單位】華東師范大學(xué)（East China Normal University）

【文獻(xiàn)中引用產(chǎn)品】

魚丙二醛（MDA）ELISA試劑盒

【關(guān)鍵詞】Antibiotic，Intestinal microbiota，Gut health，Zebrafish

【影響因子(IF)】5.98

【出版期刊】《Environmental Pollution》

【產(chǎn)品原文引用】

 Biochemical assays

In order to minimize bias, each treatment contained 30 fish for biochemical analysis. The 

whole gut contents of each fish were weighed and homogenized with 9 vol (v/w) of 0.8% physiological saline. Then, the homogenate was centrifuged at 2500×g at 4℃for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instructions. Malondialdehyde (MDA) was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Hengyuan, Shanghai). Superoxide dismutase (SOD), peroxidase (POD), reduced glutathione (GSH), acid phosphatase (ACP), and alkaline phosphatase (AKP) were measured using related commercial assay kits (Nanjing Jiancheng Institute,China). Results were recorded on a microplate reader (Epoch, BioTek, USA).

恒遠(yuǎn)產(chǎn)品文獻(xiàn):魚胰島素（Insulin）ELISA試劑盒引用文獻(xiàn)（新網(wǎng)站已發(fā)）

【文獻(xiàn)標(biāo)題】Inhibited autophagy impairs systemic nutrient metabolism in Nile tilapia

【作者】Si-Lan Han，Jing Wang，Yu-Xue Zhang，et.al

【作者單位】華東師范大學(xué)（East China Normal University）

【文獻(xiàn)中引用產(chǎn)品】

魚胰島素（Insulin）ELISA試劑盒

【關(guān)鍵詞】Autophagy，Metabolism，Homeostasis，Inflammation，Nile tilapia

【影響因子(IF)】4.0

【出版期刊】《Comparative Biochemistry and Physiology, Part A》

【產(chǎn)品原文引用】

At the end of trial, all fish were fasted overnight, six fish of each group were euthanized (MS-222 at 20 mg/L) and sampled to collect tissues to measure the molecular, protein and biochemical indexes.Hepatic triglyceride (TG), glycogen, malondialdehyde (MDA), superoxide dismutase (SOD), and serum TG, free fatty acid (FFA), and glucose were assessed by commercial kits (Jiancheng Biotech Co. China).The serum insulin was detected by ELISA kits (Hengyuan Biotech Co.China). Briefly, the total lipid of the whole fish body, liver and muscle was extracted by using chloroform/methanol (2:1, v/v) as previously described (Bligh and Dyer, 1959). Briefly, the samples were homogenized in the mixed chloroform-methanol 2:1 (vol/vol), and the samples were stored at 4 °C for 24-h extraction. Afterwards, the chloroform phase was carefully moved to a clean glass tube and dried using nitrogen, and the extracted total lipid was weighed and recorded.Whole fish protein and muscle protein 

were measured by Kjeltec? 8200(FOSS, Sweden).

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